CELL CULTURE & TISSUE ENGINEERING
Applications
3D-printing of micro-porous blood capillaries model. Adaptive porosity is based on a Voronoï diagramm, with holes ranging from 400 nm to 3 µm in width - Realized with microFAB-3D.Advanced
Microfabrication of collagen scaffolds (green) for cells culture (blue).
Courtesy of O. Stephan, (UGA, France) - Realized with microFAB-3D.Standard
Flexible copper heating pad on Kapton foil for cell culture.
Created by ferric chloride wet etching using a 1.5-µm layer of positive photoresist AZ1512HS - Realized with Smart Print UV
Flexible copper heating pad on Kapton foil for cell culture. Created by ferric chloride wet etching using a 1.5-µm layer of positive photoresist AZ1512HS - Realized with Smart Print UV
Growth of MDA-MB-231 tumor cells from biopsy in a 900-µm-wide 3D-printed protein-coated scaffold for personnalized medicine applications.
Courtesy of C. Picart, A. Rengaraj (G-INP, CEA) - Realized with microFAB-3D.Advanced
U-shape micropillars for cells trapping - Realized with Smart Print UV
Rapid Prototyping of Polymeric Nanopillars for Controlling Cell Behavior.
A. Spangenberg - IS2M (CNRS) - Scientific Reports 7, Article number: 9247 (2017) - Realized with microFAB-3D.Advanced
Heater (ITO based) – sensor (Pt based) platform for cell culture monitoring.
Courtesy of D. Peyrade, France, DTIP 2020, 1-4 - Realized with Smart Print UV
Flow streams generated by bacteria confined in 3D-printed nano-houses. H.P. Zhang - Shanghai Jiao Tong University - Lab Chip, 2015,15, 4555-4562 - Realized with microFAB-3D
Highlight
Gelatin and collagen gels are good candidates for the development of cell culture media. Collagen, by its self-association properties, forms the weft of most tissues. In a biomimetic approach, collagen combined with gelatin therefore seems an interesting candidate to reconstitute the dermis. A three-dimensional (3D) structuring of gels is presented: formation of cubic, alveolar or more open structures such as stacks of hollow cubes.
This 3D structuring is made possible by the use of a laser microprinting system marketed by Microlight3D. With this system we spatially control the cross-linking of proteins, a phenomenon that occurs at the focal point of the micro-laser.
For this process it is necessary to add to the starting medium a photoinitiator, in our case a ruthenium complex. This initiator, which allows the solidification of the medium at the desired locations, also has interesting optical properties with oxygen. Its photoluminescence increases when the amount of oxygen in its environment decreases and decreases when the amount of oxygen present nearby increases. Thus, by following the intensity of ruthenium luminescence on the 3D structures that we have manufactured and colonized by living cells we have shown that we are able to highlight locally (at cell size scales) the activity of these cells when they migrate on the gel structures or when they divide on these same 3D structures. Indeed in this case they consume oxygen which can be followed by the decrease in the fluorescence intensity of ruthenium present in 3D structures.
Olivier Stephan is a teacher-researcher in the University Grenoble Alps (UGA).
Scaffolds for cells culture 2 - O. Stephan et al. - Microfabrication of collagen scaffolds for cells culture
Testing the ruthenium sensitivity in the gelatin-collagen matrix for 02 tension changes using normoxic A (19 % O2) – hypoxic B (1% O2)